plaque. plaque [plak], medicinsk och odontologisk term, se plack. (8 av 8 ord). Vill du få tillgång till hela artikeln? Testa NE.se gratis eller Logga in 

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2017-02-03

Wolff LF , Koller NJ , Smith QT , Mathur A , Aeppli D J Clin Periodontol , 24(12):900-906, 01 Dec 1997 2015-11-27 · To explore the ultrastructure of subgingival dental plaque using high-resolution field emission scanning electron microscopy (FE-SEM) and to investigate whether extracellular DNA (eDNA) could be Se hela listan på cced.cdeworld.com Using subgingival plaque specimens from periodontitis patients as inocula, these models were able to produce diverse and pathogen-enriched microcosm biofilms which mimicked the complexity of in vivo periodontitis-associated subgingival microbiota. Collection of subgingival plaques Subgingival plaquesampleswerecollectedby asterile hand scalerandtransferred ineither one ml of sterile reduced transfer fluid (RTF) consisting of 0.01% dithiothreitol in PBS, pH 7.4 or one ml of distilled water. The samples were immediately placed at 4 C and analyzed within 4 h. Subgingival plaque samples from 259 adult patients with severe periodontitis were analyzed. P. gingivalis was detected in 111 (43%) of the 259 subgingival plaque samples by culture and in 138 (53%) samples by PCR. The sensitivity, specificity, and positive and negative predictive values of the real-time PCR were 100, 94, 94, and 100%, respectively. Define subgingival plaque.

Subgingival plaque collection

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Search for more papers by this author. Albert Salkind D.D.S. We are well aware that subgingival plaque is bathed by gingival crevicular fluid and not by saliva. However, it is essentially impossible to collect sufficient volumes of gingival fluid for coating the hydroxyapatite disks. For this reason, we elected to use unstimulated saliva.

(2003d) Subgingival plaque removal at interdental sites using a low-abrasive air-​polishing powder. Journal of Peri- odontology 74; 307-311. Petersilka G 

Subgingival plaque samples were collected as described in [35]. In brief, four subgingival biofilm  dental plaque biofilm; periodontitis; host defense mechanisms; resistance microbiota, accompanied by a diverse collection of archaea, fungi, protozoa, and viruses. The specific composition of subgingival bacterial plaque in gingiv 10 Jun 2015 subgingival plaques of Algerian healthy adults.

Subgingival plaque sample collection. For the diseased samples, the deepest three pockets were selected and pooled. Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze. The site was then cleaned and isolated using cotton roles and air dried gently.

A total of 1,170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the was followed by collection of a pooled subgingival sample, collected from four sites with the deepest PD using the curette technique.26 Subgingival plaque samples were suspended in sterile saline (500 𝜇l).

Buccal and subgingival plaque samples were collected 1 week after the full-mouth periodontal examination. All participants were requested to refrain from food for 8 h and oral hygiene (brushing or flossing the teeth) for 12 h before sampling. Samples from all subjects were collected and stored at −80°C for subsequent Plaque absorbs calcium and phosphate from saliva for the formation of supragingival calculus and from crevicular fluid for the formation of subgingival calculus. Calcium phosphate supersaturation, certain membrane-associated components, and the degradation of nucleation inhibitors are required for initial mineralization of plaque and bacteria. Pooled subgingival plaque samples were collected and analyzed for detection of bacteria and viruses using loop‐mediated isothermal amplification. Prevalence of subgingival A. actinomycetemcomitans , HCMV, and P. gingivalis were significantly higher among aggressive periodontitis patients than periodontally healthy controls.
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Subgingival plaque samples were collected as described in [35]. In brief, four subgingival biofilm  dental plaque biofilm; periodontitis; host defense mechanisms; resistance microbiota, accompanied by a diverse collection of archaea, fungi, protozoa, and viruses. The specific composition of subgingival bacterial plaque in gingiv 10 Jun 2015 subgingival plaques of Algerian healthy adults. Supragingival and subgingival plaque samples were collected from 65 Algerian caries-free,  for diagnostic quantitative anaerobic culture of subgingival plaque samples.

Subgingival plaque sample collection: Removal of supragingival plaque with sterile cotton pellets and isolation of teeth with cotton rolls was done. Subgingival plaque was then collected with sterile paper points (Meta Biomed ® Co. Ltd, Cheongju, South Korea) inserted into the gingival sulcus of Ramfjord’s index teeth for 15 seconds(7-9).
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CHARACTE -RISTICS SUPRAGINGIVAL SUBGINGIVAL LOCATION Coronal MG Apical MG ORIGIN Salivary glycoprotein down growth bact from supra gingival plaque DISTRIBU- TION Starts proximal surface and other protected areas Heaviest collection on areas not cleaned daily by patient Cervical third , esp. facial and lingual Mandibular molars Proximal surfaces Pits and fissures plaque Shallow pocket Attached

17 May 2018 Collection of subgingival biofilms and saliva.